A Promising Analytical Method has been Crafted, Validated, and Quantified for Estimating Theophylline Utilizing UV Spectroscopic and RP-HPLC Techniques in Conjunction with Stress Degradation Studies to Pinpoint Deterioration

Introduction: The current study aimed to develop and assess UV-spectrophotometric (zero order, first second area under the curve) RP-HPLC methods for estimating theophylline in its pharmaceutical dosage form.
 Methods: A less toxic solvent composition of acetonitrile 0.1% orthophosphoric acid (25:75 v/v) is used as a mobile phase diluent developing both UV-spectroscopic techniques. Shimadzu Prominence LC-20A Modular HPLC system with C18 column (250 × 4.6 mm; 5 µm) was method.
 Results: Method zero-order spectrophotometric method determining at 271 nm, correlation coefficient linearity found be 0.9958, LOD, LOQ are 0.52 1.71 µg/mL. B first-order 258 0.9983, 0.46 1.51 C second-order 218 0.9941, 0.38 1.25 D an curve 260 282 0.9978, 0.57 1.88 E determination retention time 2.814 min, 0.9923, 0.78 2.57 Studies on stress degradation show that oxidation mostly impact solutions.
 Conclusion: Theophylline can determined using proposed approach, which convenient, precise, affordable, reproducible.